中場核磁共振系統於肝臟檢體特性應用研究

Abstract

本研究使用中場式核磁共振系統進行類肝臟細針抽吸法、肝臟生檢法之檢測與應用。透過中場式核磁共振系統，在不同頻率下量測硫酸銅溶液(CuSO4)之縱向弛豫時間T1（Longitudinal relaxation time），模擬在類肝臟細針抽吸法下產生的特性是否與肝臟生檢法相呼應，並在測量肝檢體前利用硫酸銅模擬所需之體積及穩定度之狀況進行探討與研究。藉由硫酸銅之模擬與研究，進而可以應用在肝檢體(Liver Tissues)上。透過中場式核磁共振系統，進行16管腫瘤組織(Tumor Tissue)與21管正常組織(Normal Tissue)量測。類肝臟細針抽吸法中，每一檢體取重量0.009 g~0.010 g；肝臟生檢法中，每一檢體取0.075 g~0.1250 g，進行量測弛豫時間T1。透過兩重量間之T1關係，驗證使用類肝臟細針抽吸方式取肝檢體之T1來進行腫瘤分辨的可行性。兩重量的分析結果，可發現兩者同樣很容易分辨腫瘤組織與正常組織，且在靈敏度與特異度上兩者也都超過85%以上。顯示了，在類肝臟細針抽吸下進行檢測，可得到與肝臟生檢下相同的結果，將其方法應用在病患身上，可降低病患的恐懼度與不安感，且也很快能初估病患是否有罹患肝臟上面病變。相比於傳統病理組織切片，可能需要開刀取得檢體，並進行繁瑣的固定、脫水、染色、封片，在這些過程可能耗費3-7天時間，病人的傷口上明顯比本研究之方法大上許多。因此若利用取樣針取得類肝臟細針抽吸進行T1量測，不管是取檢體上，或是分析結果、觀看病理報告上，大約只需30~40分鐘左右。病人相對可以快速理解自身初步病情，且也縮短病理結果報告的時間，進而可擴大使用類肝臟細針抽吸的可行性。In this study, Mid-Field NMR system was used for the detection and application of liver-like fine needle aspiration and liver biopsy. Measure the longitudinal relaxation time T1 (Longitudinal relaxation time) of copper sulfate solution (CuSO4) at different frequencies through a midfield nuclear magnetic resonance system, and simulate whether the characteristics produced by the liver-like fine needle aspiration method are compatible with liver biopsy The method is echoed, and copper sulfate is used to simulate the required volume and stability of the liver specimen for discussion and research.With the simulation and research of copper sulfate, it can be applied to Liver Tissues. Through the midfield MRI system, 16 tubes of tumor tissues and 21 tubes of normal tissues were measured. In the liver-like fine needle aspiration method, the weight of each specimen is 0.009 g~0.010 g; in the liver biopsy method, each specimen is taken 0.075 g~0.1250 g to measure the relaxation time T1. Through the T1 relationship between the two weights, the feasibility of using liver-like fine needle aspiration to obtain T1 of liver specimens for tumor discrimination is verified. The analysis results of the two weights show that it is also easy to distinguish between tumor tissue and normal tissue, and the sensitivity and specificity of both are more than 85%. It is shown that testing under liver-like fine needle aspiration can obtain the same results as under liver biopsy. Applying the method to patients can reduce the patient’s fear and anxiety, and it can also quickly Initially assess whether the patient has liver lesions. Compared with traditional pathological tissue slices, it may be necessary to obtain a specimen with an operation, and perform cumbersome fixation, dehydration, staining, and mounting. These procedures may take 3-7 days, and the patient’s wound is significantly larger than the method in this study. Many, Therefore, if the sampling needle is used to obtain liver-like fineneedle aspiration for T1 measurement, whether it is to take samples, analyze results, or watch pathology reports, it only takes about 30-40 minutes. Patients can understand their initial condition relatively quickly, and shorten the time for pathological results reporting, thereby expanding the feasibility of using liver-like fine needle aspiration.